TopGraph is a first principles based topology predictor.

it uses empirical amino acid membrane insertion energies to predict α-helical transmembrane spans.

if you have any questions, please be in touch via the Contact Us page.

in any publication, please cite:

Elazar, A., Weinstein, J.J., Prilusky, J. and Fleishman, S.J., 2016. Interplay between hydrophobicity and the positive-inside rule in determining membrane-protein topology. Proceedings of the National Academy of Sciences, 113(37), pp.10340-10345.

The algorithm was created by Assaf Elazar, Jonathan Weinstein, and Sarel Fleishman. The original web server was built by Jaime Prilusky. The current server was built by Shi Hoch.